Purchase of three Chromatography Systems

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    "description": "Purchase of three Chromatography Systems",
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    "procurementMethodRationale": "The AKTA Pure Micro for the JP lab will be used mainly for the preparation of cryoEM samples, the AKTA Pure Micro and its corresponding accessories are specifically designed for such a purpose and is routinely used for preparation of cryoEM samples.\r\n The final, and most crucial, stage of the experiments in Kyle\u00e2\u0080\u0099s lab require that the use of liquid chromatography devices to purify very small quantities of protein complexes for further analysis by the recently installed Glacios2 cryo-electron microscope. The experiments require that the team monitor the composition of the samples, which is achieved using an on-board multi-wavelength ultraviolet light detector, and that the lab achieve very fine separation of protein samples to ensure the highest purity. The latter can only be accomplished using the AKTA Pure Micro, because the equipment has been designed to minimise hold-up volumes between valves used for sample application and processing. Finally, the device dispenses droplets as low as 8 uL, allowing the highest possible resolution in terms of sample separation.\r\n There are no market competitors for the AKTA Pure Micro.\r\n The AKTA Pure M is a larger volume liquid chromatography device optimised for semi-automated isolation of protein complexes. This device will be required for the laboratory to isolate material essential for downstream processing in the AKTA Pure Micro. The fluid integration of the workflow between the AKTA Pure M and AKTA Pure Micro means that it is necessary to purchase the AKTA Pure M alongside the AKTA Pure Micro. Interaction with both machines is standardized through a proprietary software, Unicorn 7, not transferable between devices from other manufacturers. This will facilitate consistency of pipelines and procedures between the machines, which can be directly transferred.\r\n Given the above technical reasons the purchase of two AKTA Pure Micro\u00e2\u0080\u0099s and one AKTA Pure M has been awarded directly to Cytiva.",
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